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Effects of CCPI on the expression of <t>iNOS</t> and CD206 proteins. (A) Representative Western blot bands showed the expression of iNOS and CD206 after CCPI treatment. (B,C) Quantitative analysis of the iNOS/GAPDH and CD206/GAPDH ratios in the CCPI-treated groups, respectively. Data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. Control group; *** p < 0.001 and ** p < 0.01 vs. AD model group.
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Effects of CCPI on the expression of iNOS and CD206 proteins. (A) Representative Western blot bands showed the expression of iNOS and CD206 after CCPI treatment. (B,C) Quantitative analysis of the iNOS/GAPDH and CD206/GAPDH ratios in the CCPI-treated groups, respectively. Data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. Control group; *** p < 0.001 and ** p < 0.01 vs. AD model group.

Journal: Frontiers in Aging Neuroscience

Article Title: Unraveling the anti-neuroinflammatory mechanisms of Cervus cucumis polypeptide injection in Alzheimer’s disease: insights from network pharmacology, molecular docking, molecular dynamics simulation, and experimental validation

doi: 10.3389/fnagi.2026.1797302

Figure Lengend Snippet: Effects of CCPI on the expression of iNOS and CD206 proteins. (A) Representative Western blot bands showed the expression of iNOS and CD206 after CCPI treatment. (B,C) Quantitative analysis of the iNOS/GAPDH and CD206/GAPDH ratios in the CCPI-treated groups, respectively. Data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. Control group; *** p < 0.001 and ** p < 0.01 vs. AD model group.

Article Snippet: After being blocked in 5% skim milk for 2 h at room temperature, the membranes were incubated overnight with the following primary antibodies: inducible nitric oxide synthase (iNOS) mouse antibody (CAS No. IC259554, Abmart, China, 1:1,000), CD206 mouse antibody (CAS No. ZY-5843R, Abmart, China, 1:1,000), IL-6 mouse antibody (CAS No. 66146-2, Abmart, China, 1:1,000), STAT3 mouse antibody (CAS No. YA056, Abmart, China, 1:1,000), STAT3 phosphorylation mouse antibody (CAS No. 05-485, Sigma, United States, 1:1,000), VEGF rabbit antibody (CAS No. AF1309, Abmart, China, 1:1,000) and GAPDH rabbit antibody (CAS No. 10494-1-AP, Abmart, China, 1:1,000).

Techniques: Expressing, Western Blot, Control

Comparison of the effects of CCPI and LA on IL-6 secretion, STAT3 phosphorylation, and the expression of markers associated with pro-inflammation (iNOS) and anti-inflammation/repair (CD206) in AD model cells. (A) IL-6 levels in the AD model cells were measured by ELISA. (B) Western blot showed the expression of iNOS, CD206, STAT3 phosphorylation, and STAT3 after CCPI and LA treatment. (C–E) Quantitative analysis of the iNOS/GAPDH, CD206/GAPDH, and STAT3 phosphorylation/STAT3 ratios in the CCPI and LA-treated groups, respectively. Data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001, ** p < 0.01 and * p < 0.05 vs. AD model group; ns p > 0.05 compared with the CCPI group; compared with the LA group, ns p > 0.05.

Journal: Frontiers in Aging Neuroscience

Article Title: Unraveling the anti-neuroinflammatory mechanisms of Cervus cucumis polypeptide injection in Alzheimer’s disease: insights from network pharmacology, molecular docking, molecular dynamics simulation, and experimental validation

doi: 10.3389/fnagi.2026.1797302

Figure Lengend Snippet: Comparison of the effects of CCPI and LA on IL-6 secretion, STAT3 phosphorylation, and the expression of markers associated with pro-inflammation (iNOS) and anti-inflammation/repair (CD206) in AD model cells. (A) IL-6 levels in the AD model cells were measured by ELISA. (B) Western blot showed the expression of iNOS, CD206, STAT3 phosphorylation, and STAT3 after CCPI and LA treatment. (C–E) Quantitative analysis of the iNOS/GAPDH, CD206/GAPDH, and STAT3 phosphorylation/STAT3 ratios in the CCPI and LA-treated groups, respectively. Data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001, ** p < 0.01 and * p < 0.05 vs. AD model group; ns p > 0.05 compared with the CCPI group; compared with the LA group, ns p > 0.05.

Article Snippet: After being blocked in 5% skim milk for 2 h at room temperature, the membranes were incubated overnight with the following primary antibodies: inducible nitric oxide synthase (iNOS) mouse antibody (CAS No. IC259554, Abmart, China, 1:1,000), CD206 mouse antibody (CAS No. ZY-5843R, Abmart, China, 1:1,000), IL-6 mouse antibody (CAS No. 66146-2, Abmart, China, 1:1,000), STAT3 mouse antibody (CAS No. YA056, Abmart, China, 1:1,000), STAT3 phosphorylation mouse antibody (CAS No. 05-485, Sigma, United States, 1:1,000), VEGF rabbit antibody (CAS No. AF1309, Abmart, China, 1:1,000) and GAPDH rabbit antibody (CAS No. 10494-1-AP, Abmart, China, 1:1,000).

Techniques: Comparison, Phospho-proteomics, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Control